Ethidium bromide: a nucleic acid stain for tissue section.
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چکیده
منابع مشابه
Method to reduce the quantity of ethidium bromide required to stain DNA in agarose gels.
Ethidium bromide-agarose gel electrophoresis is an extensively used procedure for the analysis of DNA; it has been reported to be sensitive to as little as 50 ng of DNA (7). The standard methods for staining gels involve adding 0.5 μg/mL ethidium bromide to both the agarose gel and the running buffer or soaking the gel in a solution of ethidium bromide after electrophoresis has been performed (...
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Ethidium bromide has been extended by fusing an additional aromatic ring resulting in a larger intercalator with increased affinity for poly r(A) x r(U), poly d(A) x d(T) and triple helices when compared to the parent heterocycle.
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Molecular Probes prepares the most extensive assortment of nucleic acid stains commercially available, many of which have been developed in our research laboratories. This section discusses the physical properties of the various classes of dyes, listed below. The sections in Chapter 8 that follow discuss numerous applications of these dyes and our other reagents and technology for genomics rese...
متن کاملMechanism of Ethidium Bromide Fluorescence Enhancement on
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclu...
متن کاملEthidium bromide-resistant mutant of Bacillus subtilis.
An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 mug of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and p...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1981
ISSN: 0022-1554,1551-5044
DOI: 10.1177/29.4.6166660